I’m a second year medical student in India and confused about some techniques in Biochemistry practicals. Can someone explain how to prepare standard solutions and perform calorimetric estimations properly?
For colorimetric estimations, you need to make a standard curve using standard solutions of known concentrations. Plot the absorbance on the y-axis and concentration on the x-axis. The concentration of your unknown sample can then be determined from the standard curve.
Don’t forget to include a blank for calibration. Also be very precise while pipetting reagents to get accurate results. Proper dilution of samples is important as well. Let me know if you have any other specific doubts.
Lol it’s just making solutions , how hard can it be? Stop stressing over small things and focus on actually learning the theory. Practicals are just for show.
Author
Posts
Viewing 5 posts - 1 through 5 (of 5 total)
You must be logged in to reply to this topic.
Manage push notifications
We would like to show you notifications for the latest news and updates.
You are subscribed to notifications
We would like to show you notifications for the latest news and updates.
.
.
